General Cores:
The Molecular/Histology Core is fully outfitted
to perform any tissue processing needed, from routine H&E to immunocytochemistry to in situ
hybridization (Leica TP1020 tissue processor, Leica EG1160 embedding center, Leica CM3050S
cryostat, BioRad 550 microplate reader). A variety of light and fluorescent microscopes under
computer control (Nikon E800, DIAPHOT300 inverted stage, MEIJI) can acquire data automatically
and store arrays for offline analysis with custom designed software. Cell culture facilities
enable advanced contrast testing in vitro on target cell types. Routine molecular analyses
(rtPCR, etc.) can be performed as required with a Real Time PCR: Model 7300 instrument
(Applied Biosystems). CyAn ADP High-Performance Flow Cytometer: The CyAn ADP Analyzer is the
next step in advanced digital processing with enhanced detection for flow cytometry. The CyAn
ADP has state-of-the-art optics, electronics, and fluidics offering automated compensation
with the highest acquisition rates. Our model accommodates a diverse range of applications,
with 9 standard parameters and 7 colors. Biacore X100: The Biacore X100 provides label-free
protein interaction analysis leading to unique and comprehensive data on the interactions
between biomolecules. It’s features include: Quantitative kinetic analysis (rate constants),
quantitative determination of affinity constants, determination of binding specificity (yes/no),
concentration analysis. Various freezers, centrifuges, and other prep and storage equipment
also are on site.
The Clinical Core encompasses HIPPA compliant
processes for recruiting and followup of patients enrolled in experimental trials of new
imaging procedures and devices, new drug studies, and normal volunteers for test
development. Two full time nurses contact and recruit patients, maintain patient
and study files, and ensure compliance. Reports (research) are generated with a
custom-designed searchable data base and reporting tool for each studies and supplied to
physicians as requested. Confidentiality is maintained according to IRB and HIPAA
guidelines. Comfortable patient waiting and interview areas at the
entrance to the CAN enable easy access to the imaging suites.